用广州血管圆线虫成虫切制成 4 μm厚的冰冻切片作抗原,用间接荧光抗体试验检测感染广州血管圆线虫大鼠血清的抗体,结果在感染后 2 wk的 50份鼠血清中,呈阳性反应46份,阳性率为 92.0％;在感染后 4 wk的 19份鼠血清中,呈阳性反应 19份,阳性率为 100％;对照的阴性血清及盐水均呈阴性反应。本法检出率高,且感染早期出现阳性反应,可作广州血管圆线虫病早期辅助诊断。 The frozen sections of 4 μm in diameter were harvested from the adult C. elegans of Guangzhou and used as the antigen. The antibodies against the serum of C. elegans were detected by indirect fluorescent antibody test. The results showed that the antibodies were positive in 50 rat serums 2 weeks after infection 46 were positive, the positive rate was 92.0%. In 19 rat sera 4 weeks after infection, 19 were positive, the positive rate was 100%. The negative serum and saline of the control were negative. The detection rate of this law is high, and positive infection early, can be used for early diagnosis of angiostrongyliasis in Guangzhou.