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用热水处理蔗髓,通过控制水解温度使L-阿拉伯糖选择性水解以利于L-阿拉伯糖的提取。考察了温度、水解时间及液比对蔗髓水解产出L-阿拉伯糖、木糖及葡萄糖的影响。采用高效液相色谱法对水解液中的单糖含量进行了检测,结果表明:温度对水解液组分的影响最为显著,当温度较低(≤140℃)时,L-阿拉伯糖有明显溶出,而木糖与葡萄糖溶出很少甚至不溶出;当温度超过160℃,木糖产率大于L-阿拉伯糖与葡萄糖;在较低温度下延长保温时间能够使L-阿拉伯糖有较大量溶出,同时木糖及葡萄糖溶出很少。实验得到蔗髓水解提取L-阿拉伯糖的适宜条件为:水解温度140℃,保温时间3h,液比1∶10,此时L-阿拉伯糖的产率为1.40%,木糖、葡萄糖未检出。
The pith is treated with hot water, and L-arabinose is selectively hydrolyzed by controlling the hydrolysis temperature to facilitate the extraction of L-arabinose. The effects of temperature, hydrolysis time and liquid ratio on the hydrolysis of L-arabinose, xylose and glucose by peanut pulp were investigated. The content of monosaccharide in the hydrolyzate was tested by HPLC. The results showed that temperature had the most significant influence on the composition of the hydrolyzate. When the temperature was low (≤140 ℃), the content of L-arabinose was significantly , While xylose and glucose eluted with little or no dissolution; when the temperature exceeds 160 ℃, xylose yield is greater than L-arabinose and glucose; prolong holding time at a lower temperature can make a larger amount of L-arabinose dissolution, At the same time xylose and glucose leaching rarely. The optimal conditions for the extraction of L-arabinose from the hydrolyzed peanut pulp were as follows: hydrolysis temperature 140 ℃, incubation time 3h, liquid ratio 1:10, the yield of L-arabinose 1.40%, xylose, glucose not detected .