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研究了在赤霉病菌粗毒素作用下,西农67(抗病品种)和8433(感病品种)小麦幼苗MDA含量,SOD、POD活性及其同工酶变化。结果表明,西农67小麦幼苗在粗毒素作用24h内,SOD和POD活性迅速增加,此后,随着毒素作用时间的延长,这两种酶能够维持高的活性水平,其MDA累积缓慢,累积量少;感病品种8433在粗毒素作用下,SOD和POD活性均呈先增加后降低的趋势.且两种酶活性增加幅度均小于西农67,其MDA则迅速累积,累积量大于西农67。同工酶的研究结果表明,西农67比8433多出一条SOD同工酶带,两个品种POD同工酶谱相同;经粗毒素处理后.两个品种SOD同工酶谱变化主要是同工酶带1和酶带4活性变化,POD同工酶谱有新酶带出现,西农67增加2条新酶带(酶带6和8).8433增加1条(酶带6)。
The activities of MDA and the activities of SOD and POD and the isozymes of wheat seedlings of Xiong 67 (resistant varieties) and 8433 (susceptible cultivars) were studied under the action of crude toxin of Fusarium graminearum. The results showed that the activities of SOD and POD in wheat seedlings of Xixin 67 increased rapidly within 24h after the toxins were treated, and the activities of SOD and POD increased rapidly with the prolongation of the time of toxins. Less; susceptible variety 8433 under the action of the crude toxin, SOD and POD activity were increased first and then decreased. The increase of both activities was less than that of Xinong 67, and its MDA accumulated rapidly with a cumulative amount greater than that of Xinong 67. Isoenzyme results showed that more than one of the 8433 Xinnong 67 isozyme bands, the same POD isozyme profiles of the two varieties; the crude toxin treatment. The changes of SOD isozymes of two cultivars were mainly the changes of activities of isozymes bands 1 and 4 and the emergence of new bands of POD isozymes. Two new bands of enzymes (bands 6 and 8) . 8433 Add 1 (Enzyme Band 6).