目的:采用高效液相色谱法,建立9种不同提取工艺的复方“韵通”特征图谱。方法:色谱条件:Venusil XBP C18(L)(4.6 mm×250 mm),检测波长:283 nm,柱温:25℃,流动相:乙腈(10%～100%)︰0.2%磷酸溶液(0～90%),梯度洗脱70min。结果:通过分析,确立了不同工艺提取复方“韵通”特征图谱的17个共有峰,主要成分黄芩苷含量不少于0.60%。结论:该方法的精密度,稳定性,重复性良好,可用于复方“韵通”药材及其制剂的质量控制,为临床应用的选择提供参考。 OBJECTIVE: To establish a compound “rhyme ” characteristic map of nine different extraction processes by high performance liquid chromatography. The chromatographic conditions were as follows: Venusil XBP C18 (L) (4.6 mm × 250 mm), detection wavelength of 283 nm, column temperature of 25 ℃, mobile phase of acetonitrile 10% -100% 90%), gradient elution 70min. Results: Through the analysis, 17 common peaks were obtained by different techniques to extract the compound “Rhyme” characteristic map with the content of baicalin not less than 0.60%. Conclusion: The precision, stability and repeatability of this method are good and can be used for the quality control of the compound “Yun Tong” and its preparation, which can provide a reference for the choice of clinical application.