论文部分内容阅读
高水平抗纹枯病突变体和高感纹枯病品种蜀恢881杂交构建分离群体,经F2分离世代的遗传分析,抗、感单株比例符合3 1(χc2=0.563,χ12,0.05=3.84),初步确定该突变体对纹枯病的抗性由一对显性主效基因所控制,命名为Rsb-2(t)。利用已合成的530对微卫星引物,对抗纹枯病突变体和蜀恢881进行多态性引物筛选,用多态性引物对上述F2分离群体的全部感病单株和部分抗病单株的DNA进行PCR分析,借助MAPERMAKER/EXP3.0软件,对其微卫星标记实验数据进行连锁分析,将Rsb-2(t)定位于第3染色体的p臂,发现RM218、RM251、RM4321和RM5748与Rsb-2(t)连锁,它们均位于着丝粒端,连锁距离分别为32.1 cM,41.1 cM,42.4 cM和49.7 cM。研究结果为进一步对该基因的精细定位奠定了基础。
High-level resistance to sheath blight mutants and susceptible blight variety Shuhui 881 were used to construct segregant populations. Genetic analysis showed that the resistant and susceptible plant lines were segregated by 3 1 (χc2 = 0.563, χ12, 0.05 = 3.84 ), Preliminary identified that the resistance of the mutant to sheath blight was controlled by a dominant dominant gene named Rsb-2 (t). A total of 530 pairs of microsatellite primers were synthesized to screen for the resistance to sheath blight mutants and Shuhui 881, and the polymorphic primers were used to screen all the susceptible and partial resistant plants DNA was analyzed by PCR, and the microsatellite markers were analyzed by linkage analysis with MAPERMAKER / EXP3.0 software. Rsb-2 (t) was located on the p-arm of chromosome 3 and found that RM218, RM251, RM4321 and RM5748 interacted with Rsb -2 (t), all located at the centromeric end, with linkage distances of 32.1 cM, 41.1 cM, 42.4 cM and 49.7 cM, respectively. The results laid a foundation for further fine mapping of this gene.