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目的:采用HPLC法同时测定五味子中3种木脂素类成分(五味子醇甲、五味子甲素、五味子乙素)含量。方法:KromasilTMC18柱(250mm×4.6mm,5μm);流动相:甲醇0.05%磷酸水溶液(80∶20),流速1.0mL/min;柱温25℃,检测波长250nm。结果:五味子醇甲、五味子甲素、五味子乙素3种成分的线性范围分别是191.8~2685.2ng、664~4648ng、356~4984ng;平均加样回收率分别为99.98%、100.37%、100.03%;三者RSD分别为0.68%、0.98%、1.06%。结论:该方法精密度、重复性和分离效果好,分析快速准确,可用于五味子药材的质量控制,并且可以作为目前五味子国家标准的有益补充。
OBJECTIVE: To determine the contents of three lignan components (schizandrin, schisandrin and schisandrin B) in Schisandra chinensis by HPLC method. Methods: Kromasil TMMC18 column (250mm × 4.6mm, 5μm) was used. The mobile phase was methanol 0.05% phosphoric acid (80:20) at a flow rate of 1.0mL / min. The column temperature was 25 ℃ and the detection wavelength was 250nm. Results: The linear ranges of schizandrin, schizandrin and schisandrin B were 191.8 ~ 2685.2ng, 664 ~ 4648ng and 356 ~ 4984ng, respectively. The average recoveries were 99.98%, 100.37% and 100.03%, respectively. The three RSD were 0.68%, 0.98%, 1.06%. Conclusion: The method has good precision, repeatability and separation effect, rapid and accurate analysis, and can be used for the quality control of Schisandra sp., And can be used as a useful supplement to the current national standards of Schisandra.