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过敏性哮喘(AA)与非过敏性哮喘(NAA)的临床征象不尽相同。T细胞亚群及其产生的细胞活素可能与不同类型哮喘的发病机制有关。通过对AA和NAA患者周围血及支气管肺泡灌洗液(BALF)中T细胞亚群及其有关的细胞活素的检测,探讨T细胞在哮喘免疫病理学中的作用。方法 AA和NAA两组患者各35例,均按美国胸腔学会标准诊断;正常对照50例。分离和培养周围血和BALF(每组各10例)中T细胞,收集细胞培养上清液和BALF。应用免疫荧光法鉴定T细胞亚群,用酶联免疫吸附试验或生物检测法测定上清液中的细胞活素。
Allergic asthma (AA) and non-allergic asthma (NAA) clinical signs are not the same. T-cell subsets and their production of cytokines may be involved in the pathogenesis of different types of asthma. T cell subsets and their related cytokines in peripheral blood of patients with AA and NAA and bronchoalveolar lavage fluid (BALF) were assayed to explore the role of T cells in the immunopathology of asthma. Methods AA and NAA 35 patients in both groups were diagnosed according to American Thoracic Society standard; 50 cases of normal control. T cells were isolated and cultured from peripheral blood and BALF (10 in each group), and cell culture supernatant and BALF were collected. T cell subsets were identified by immunofluorescence and cytokines in supernatants were determined by enzyme-linked immunosorbent assay (ELISA) or bioassay.