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以红丰11为轮回亲本、Clark为供体亲本构建回交群体进行耐旱性鉴定,对获得选择群体进行全基因组SSR标记扫描,计算供体基因型导入频率,利用卡方测验检测偏分离SSR位点,并结合GGT软件对各连锁群分析,对5个耐旱相关性状进行QTL定位。以卡方测验检测到23个SSR偏分离位点(超导入),分布于10条连锁群。方差分析表明,8个叶片持水能力QTL分布于A1、B1、C2、E、L和N连锁群;9个根长QTL分布于C2、F、G和I连锁群;11个根干重QTL分布于A2、B1、B2、E、F、K、L、M和O连锁群;12个产量QTL分布于B1、D1a、E、F、G、I、L、M和O连锁群;7个生物量QTL分布于E、F、G、K、L和N连锁群。在E连锁群的Sat_136位点,对于叶片持水能力、根干重、产量和生物量具有一致性;在F连锁群的GMRUBP位点,对于根干重和生物量具有一致性,Satt586位点,对于根长、根干重和产量具有一致性;在K连锁群的Satt167位点,对于根干重和生物量具有一致性,SOYPRP1位点,对于根长和生物量具有一致性;在L连锁群的Satt398位点,对于根长和产量具有一致性,Satt694位点对于叶片持水能力和生物量具有一致性;在M连锁群的GMSL514位点,对于根干重和产量具有一致性;以上位点均与卡方测验检测到的“超导入”位点具有一致性。经过供体等位基因卡方测验和耐旱QTL定位,共检测到33个QTL,其中有17个同时被检测到。这些位点可能是控制大豆耐旱性的重要位点。
Using Hongfeng 11 as the reincarnation parent, Clark constructed the backcross population for the donor parents for drought tolerance identification. Whole genome SSR markers were screened for the selected populations, and the frequencies of donor genotypes were calculated. The chi-square test was used to detect the SSR Site, and combined with GGT software for each linkage group analysis, QTL mapping of five drought-resistant traits. Twenty-three SSR segregation sites (superconductivity) were detected by chi-square test and distributed in 10 linkage groups. Analysis of variance showed that the QTLs for water holding capacity of eight leaves distributed in A1, B1, C2, E, L and N linkage groups; 9 root length QTLs were located in linkage groups C2, F, G and I; 12 QTLs were distributed in B1, D1a, E, F, G, I, L, M and O linkage groups; 7 QTLs were distributed in A2, B1, B2, E, F, K, Biomass QTLs were distributed in E, F, G, K, L and N linkage groups. At the Sat_136 site of E-linkage group, there was consistency in leaf water holding capacity, root dry weight, yield and biomass. At the GMRUBP site of F linkage group, there was consistency in root dry weight and biomass, Satt586 site , And there was consistency between the root length and root dry weight and yield. At the Satt167 locus of K linkage group, there was consistency between root dry weight and biomass, and SOYPRP1 locus was consistent with root length and biomass. The Satt398 locus in the linkage group was consistent with root length and yield. Satt694 locus was consistent with leaf water holding ability and biomass. At the GMSL514 locus in M linkage group, the root dry weight and yield were consistent. The above sites were consistent with the “superconducted” sites detected by the chi-square test. After the donor allele chi-square test and drought-tolerant QTL mapping, a total of 33 QTLs were detected, of which 17 were detected simultaneously. These sites may be an important site to control soybean drought tolerance.