论文部分内容阅读
目的观察中药超微粉腧穴敷贴对变应性鼻炎大鼠血清IFN-γ和IL-4含量的影响。方法 50只SD大鼠随机分为正常对照组和造模组,造模组给予卵蛋白腹腔注射、滴鼻及喷雾吸入,造模成功后再随机分为空白模型组(A组)、丙酸氟替卡松组(B组)、普通粉组(C组)和超微粉组(D组)。干预10天后,采集外周血ELISA法测IL-4和IFN-γ。结果1A组血清IFN-γ含量较正常对照组大幅降低,B组、C组和D组血清IFN-γ含量较A组显著升高(P<0.05),D组血清IFN-γ含量与正常对照组相比无统计学差异(P=0.584);2A组血清IL-4含量较正常对照组大幅升高,B组、C组和D组血清IL-4含量较A组显著降低(P<0.05),D组血清IL-4含量与正常对照组及B组相比无统计学差异(P=0.095、0.07)。结论超微粉腧穴敷贴能显著升高IFN-γ和降低IL-4,提示其可能的作用机制是恢复Th1和Th2细胞的平衡关系。
Objective To observe the effect of superfine acupoint injection of Chinese medicine on serum levels of IFN-γ and IL-4 in allergic rhinitis rats. Methods Fifty Sprague-Dawley rats were randomly divided into normal control group and model group. The model group was given intraperitoneal injection of ovalbumin, nasal drops and inhalation spray. After successful modeling, they were randomly divided into blank model group (group A), propionic acid Fluticasone group (group B), common powder group (group C) and ultrafine powder group (group D). After 10 days of intervention, IL-4 and IFN-γwere collected by ELISA. Results The level of serum IFN-γ in group 1A was significantly lower than that in control group. The levels of IFN-γ in group B, C and D were significantly higher than those in group A (P <0.05) (P = 0.584). Serum levels of IL-4 in group 2A were significantly higher than those in control group. The levels of IL-4 in group B, group C and group D were significantly lower than those in group A (P <0.05) ). There was no significant difference in serum IL-4 level between group D and normal control group and group B (P = 0.095,0.07). CONCLUSION: Application of ultrafine acupoints can significantly increase IFN-γ and IL-4, suggesting that its possible mechanism is to restore the balance between Th1 and Th2 cells.