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In order to ensure the safety of the non-heating gathering and transportation processes for high water fraction crude oil, the effect of temperature, water fraction, and flow rate on the flow characteristics of crude oil with high water fraction was studied in a flow experimental system of the X Oilfield. Four distinct flow patterns were identified by the photographic and local sampling techniques. Especially, three new flow patterns were found to occur below the pour point of crude oil, including EW/O&W stratified flow with gel deposition, EW/O&W intermittent flow with gel deposition, and water single-phase flow with gel deposition. Moreover, two characteristic temperatures, at which the change rate of pressure drop had changed obviously, were found during the change of pressure drop. The characteristic temperature of thefirst congestion of gel deposition in the pipeline was determined to be the safe temperature for the non-heating gathering and transportation of high water cut crude oil, while the pressure drop reached the peak at this temperature. An empirical formula for the safe temperature was established for oil-water flow with high water fraction/low fluid production rate. The results can serve as a guide for the safe operation of the non-heating gathering and transportation of crude oil in high water fraction oilfields.