论文部分内容阅读
妊娠第10~11和第14~15天的BALB/C小鼠被麻醉后,经腹正中切口将子宫角外置于腹壁上。右、左侧子宫角近卵巢的第一或第二个着床点分别定为辐照组或假照组。用0.8MHz连续超声波分别以五种强度(Ow/cm~2一假照;8W/cm~2,12W/cm2,16W/cm~2,20W/cm~2)辐照妊娠鼠的158个着床点,实时观察心电变化后,还纳子宫,关腹,于妊娠第18天时检查。另有60只妊娠鼠作超声热效应研究。结果:心电消失后未再出现组中,胚胎的死亡率为100%,在妊娠第10~11天组中超声波致胚胎急性完全性死亡剂量为20W/cm~2,61秒;16W/cm2,101秒;而妊娠第14~15天组的剂量则是:20W/cm~2,68秒。用12W/cm2,16W/cm~2和20W/cm~2三组声强辐照妊娠第14天胚胎着床点90秒,胚胎体内温度平均升高6.88℃,11.79℃和26.59℃。
BALB / C mice from the 10th to 11th and 14th to 15th days of gestation were anesthetized, and the uterus was placed on the abdominal wall by trans-abdominal median incision. The first or second implantation site of the right and left uterine horns near the ovary were designated as irradiation group or sham group, respectively. A total of 158 irradiated pregnant rats were irradiated with 0.8MHz continuous ultrasound at five intensities (Ow / cm ~ 2 for a sham; 8W / cm ~ 2,12W / cm2,16W / cm2 ~ 20,20 / cm2) Implantation point, real-time observation of changes in ECG, but also to accept the uterus, abdomen, in the first 18 days of pregnancy check. Another 60 pregnant rats for ultrasound effects. Results: The mortality of embryos was 100% in the group without ECG reappearance after ECG disappeared. The acute complete death dose caused by ultrasound was 20W / cm ~ 2, 61 seconds in the group of 10 ~ 11 days of gestation; , 101 seconds; while the first 14 to 15 days of pregnancy dose is: 20W / cm ~ 2,68 seconds. With the intensity of 12W / cm2, 16W / cm ~ 2 and 20W / cm ~ 2, the embryo implantation sites on the 14th day of pregnancy were irradiated for 90 seconds. The average body temperature in the embryos increased 6.88 ℃, 11.79 ℃ and 26 .59 ° C.