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目前有大量关于全程电荷匹配设计肽的应用的研究,但是关于半程电荷匹配设计肽作为药物的应用研究却未见报道.本文对新型设计的半程电荷匹配肽P9(AC-Pro-Ser-Phe-Asn-Phe-Lys-Phe-Glu-Pro-NH2)的研究发现,P9可以成功地将作为疏水药物模型的芘稳定悬浮于水溶液中.大豆卵磷脂微囊被用以模拟细胞膜.荧光数据显示芘可以借助P9的包埋作用以晶体状态存在于水溶液中.并且当包埋体加入到脂质体微囊体系中时,芘可以从P9的包埋体系中以分子形态释放到膜的脂质层内.实验发现当包埋于芘上的P9较多,包埋壁较厚,芘的释放速度相对变缓.我们可以利用这一特性,通过调节加入的P9的浓度来控制包埋于芘上P9的厚度,从而控制芘的释放速率.研究表明,具有半程电荷匹配性质的P9肽可以作为运载疏水药物的载体.
There are a lot of studies on the application of the full charge matching design peptide, but there is no report on the application of the half charge matching design peptide as the drug.In this paper, the new design of the half charge matching peptide P9 (AC-Pro-Ser- Phe-Asn-Phe-Lys-Phe-Glu-Pro-NH2) found that P9 can successfully suspend pyrene as a hydrophobic drug in aqueous solution.Soy lecithin microcapsules were used to simulate the cell membrane.Fluorescence data Pyrene can be shown to be in crystalline state in aqueous solution by the embedding of P9 and pyrene can be released molecularly from the embedding system of P9 to the lipid of the membrane when it is incorporated into the liposomal microcapsule system In the stratum, we found that when the P9 embedded in pyrene is more and the embedding wall is thicker, the release rate of pyrene is relatively slow.We can use this characteristic to control the concentration of P9 Pyrene on the thickness of P9, thereby controlling the release rate of pyrene.Studies have shown that, with half the charge-matching properties of P9 peptide can be used as a carrier for carrying hydrophobic drugs.