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应用改良的Ｆｅｎｔｏｎ方法由出生２４小时内的新生Ｗｉｓｔａｒ大鼠分离破骨细胞（ＯＣ）培养于盖玻片与骨片上，并应用改良的ＶａｎＤｅＷｉｊｎｇａｅｒｔ和Ｍｏｓｔａｆａ方法对培养在骨片上的ＯＣ进行ＴＲＡＰ染色，观察体外培养ＯＣ的形态与生存时间。培养于骨片上的ＯＣ生存时间可长达２４０小时。１×１０（－９）ｍｏｌ／Ｌ鳗鱼降钙素能明显减少体外培养的多核ＯＣ生存数，但对单核前ＯＣ数没有明显影响
The osteoclasts (OC) were isolated from newborn Wistar rats within 24 hours after birth by modified Fenton method and then cultured on coverslips and bone slices. TRAP staining was performed on osteoclasts cultured with modified VanDeWijngaert and Mostafa methods. Observed in vitro OC morphology and survival time. OC cultured on bone chips can survive for up to 240 hours. 1 × 10 (-9) mol / L eel calcitonin significantly reduced the number of multi-nuclear OC in vitro culture, but had no significant effect on the number of pre-mononuclear OC