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目的制备并分离纯化PEG修饰的腺病毒-阴离子脂质体复合物。方法采用薄膜分散法制备空白阴离子脂质体,利用钙离子融合法制备阴离子脂质体与腺病毒的复合物,最后用后插入法制备PEG修饰的腺病毒-阴离子脂质体复合物。通过蔗糖密度梯度离心法分离制备的复合物,分别测定分离得到的各组分中脂质体、PEG-脂质材料及腺病毒的含量。结果成功制备了PEG修饰的腺病毒-阴离子脂质体复合物,连接到脂质体上的PEG-脂质占加入总量的28.9%,被脂质体包裹的腺病毒占加入量的35.9%。结论通过蔗糖密度梯度离心可得到纯化的PEG修饰的腺病毒-阴离子脂质体复合物。
Objective To prepare and separate purified PEG-modified adenovirus-anionic liposome complexes. Methods Blank anionic liposomes were prepared by membrane dispersion method. The complexes of anionic liposomes and adenovirus were prepared by calcium ion fusion method. Finally, PEG modified adenovirus - anionic liposome complexes were prepared by post insertion method. The prepared complexes were separated by sucrose density gradient centrifugation, and the contents of liposomes, PEG-lipid materials and adenoviruses were separately determined. Results The PEG-modified adenovirus-anionic liposome complexes were successfully prepared. PEG-lipids attached to the liposomes accounted for 28.9% of the total amount, liposome-encapsulated adenovirus accounted for 35.9% . Conclusion Purified PEG-modified adenovirus-anionic liposome complexes were obtained by sucrose density gradient centrifugation.