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目的 研究全反式维甲酸(ATRA)对培养的大鼠动脉平滑肌细胞(VSMCs)的影响。方法 血管平滑肌细胞采用组织贴块法培养。ATRA(2.5×10-6mol/L)分别作用于经PDGF—BB(10 ng/ml)和20％FCS刺激后的VSM—Cs,作用24h后分别行3H—TdR,3H—Leucine掺入实验测定。结果 ATRA明显抑制由PDGF—BB(10ng/ml)和20％FCS促进的VSMCs的DNA和蛋白质合成。结论ATRA具有抑制VSMCs增殖的作用。
Objective To investigate the effect of all-trans retinoic acid (ATRA) on cultured rat arterial smooth muscle cells (VSMCs). Methods Vascular smooth muscle cells were cultured by tissue patch method. ATRA (2.5 × 10-6mol / L) was applied to VSM-Cs treated with PDGF-BB (10 ng / ml) and 20% FCS for 24h and then 3H-TdR and 3H-Leucine incorporation assay . Results ATRA significantly inhibited DNA and protein synthesis of VSMCs promoted by PDGF-BB (10 ng / ml) and 20% FCS. Conclusion ATRA can inhibit the proliferation of VSMCs.