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在内蒙古高原荒漠区依照红砂的自然分布,按湿润度梯度,选取了4个样点进行考察、取样,应用ISSR分子标记,对红砂种群的遗传多样性进行研究.结果表明:14条ISSR引物扩增出307个条带,多态位点百分率为99.02%,揭示了红砂基因组具有丰富的多态性.4个红砂种群Nei遗传多样性指数的变化范围在0.1962~0.2392,Shannon信息指数的变化范围在0.3006~0.3658.分子变异分析表明,在总的遗传变异中有27.67%的变异发生在群体间,有72.33%的变异发生在群体内,种群间有高度的遗传分化.分布在极度干旱的典型荒漠中的红砂种群与其他种群的遗传距离最大,群落组成单一,具有特殊性和脆弱性,应重视保护与合理利用.
According to the natural distribution of red sand in the plateau desert area of Inner Mongolia, four samples were selected to investigate, sampling and applying ISSR markers to study the genetic diversity of R. soongorica.The results showed that 14 ISSR A total of 307 bands were amplified by PCR and the percentage of polymorphic loci was 99.02%, which revealed rich polymorphism of Neisseria gonorrhoeae genome.The Nei’s genetic diversity index of four red sand populations varied from 0.1962 to 0.2392, Shannon’s information The index ranged from 0.3006 to 0.3658. Molecular variance analysis showed that 27.67% of the total genetic variation occurred in the population, 72.33% of the variation occurred in the population, and there was a high degree of genetic differentiation among the populations In the extremely arid typical desert, the population of R. soongorica has the most genetic distance with other populations, the community has a single composition with particularity and fragility, and the protection and rational utilization should be emphasized.