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结构光照明荧光显微术(SIM)是一种可突破阿贝衍射极限的宽场显微成像技术,因其非侵入、成像速度快及光损伤小等优点在生物医学研究中具有广泛的应用前景。从结构光照明显微成像系统基本原理出发,分析了超分辨图像重构算法原理、重构图像中伪影来源及优化方法;结合研制的线性/非线性结构光照明显微镜,详细讨论了基于激光干涉的SIM成像系统光机结构。重点讨论了系统的同步时序设计和光路中的几个关键技术问题。设计对比实验验证了自主开发的SIM重构算法的可靠性,并基于研制的线性SIM系统开展细胞骨架的成像实验。最后,对SIM技术在生物上的发展和应用提出展望。
Structured Light Illumination Fluorescence microscopy (SIM) is a widefield microscopic imaging technique that can break the Abbe diffraction limit. It is widely used in biomedical research due to its advantages of non-invasiveness, fast imaging speed and small light damage. prospect. Based on the basic principle of structured light illumination microscopy system, the principle of super-resolution image reconstruction algorithm, the source of artifacts in the reconstructed image and the optimization method are analyzed. Combining the developed linear / nonlinear structured light illumination microscope, Interfering SIM Imaging System Optomechanical Structure. It focuses on the system synchronization timing design and several key technical issues in the optical path. The contrast experiments were carried out to verify the reliability of self-developed SIM reconstruction algorithm and to carry out cytoskeletal imaging experiments based on the developed linear SIM system. Finally, the prospect and development of SIM technology in biology are put forward.