论文部分内容阅读
目的:观察皮肤成纤维细胞在某些条件下释放的细胞因子对肝细胞急性期反应的影响。方法：用细菌脂多糖孵育人的皮肤成纤维细胞，测定培养液中IL－1β和IL－6浓度；并用不同浓度和组合的细胞培养液、重组IL－6（rhIL－6）和地塞米松孵育鼠肝瘤H4细胞，观察白蛋白、α1酸糖蛋白、α1抗胰蛋白酶和转铁蛋白mRNA表达的变化。结果：发现细菌脂多糖刺激成纤维细胞合成IL－6，而地塞米松抑制这一作用。IL－6和细菌脂多糖孵育的成纤维细胞培养液抑制白蛋白mRNA的表达，成纤维细胞培养液。地塞米松促进α1酸糖蛋白、α1抗胰蛋白酶mRNA的表达、单用IL－6即能抑制白蛋白和转铁蛋白mRNA的表达，地塞米松加强IL－6和成纤维细胞培养液的抑制作用。结论：（1）细菌脂多糖处理的成纤维细胞能够分泌IL－6，但不分泌11－1β；（2）成纤维细胞产生的细胞因子能够调节急性期反应时的肝脏蛋白合成；（3）地塞米松抑制成纤维细胞产生IL－6，但加强其刺激急性期反应的能力。
OBJECTIVE: To observe the effect of cytokines released by skin fibroblasts under certain conditions on the acute phase response of hepatocytes. Methods: Human dermal fibroblasts were incubated with bacterial lipopolysaccharide (LPS), and the concentrations of IL-1β and IL-6 in the culture medium were measured. The cells were treated with different concentrations and combinations of cell culture medium, rhIL-6 and dexamethasone The H4 cells were incubated with H4 cells to observe the changes of albumin, α1 acid glycoprotein, α1 antitrypsin and transferrin mRNA expression. RESULTS: Bacterial lipopolysaccharide was found to stimulate fibroblasts to synthesize IL-6, whereas dexamethasone suppressed this effect. Fibroblasts incubated with IL-6 and bacterial lipopolysaccharide inhibited albumin mRNA expression in fibroblast culture medium. Dexamethasone can promote the expression of α1 acid glycoprotein and α1 antitrypsin mRNA, and inhibit the expression of albumin and transferrin mRNA with IL-6 alone. Dexamethasone enhances the inhibition of IL-6 and fibroblast culture medium effect. Conclusions: (1) Fibroblasts treated with bacterial lipopolysaccharide can secrete IL-6, but do not secrete 11-1β. (2) Cytokines produced by fibroblasts can regulate liver protein synthesis during acute phase reaction. (3) Dexamethasone inhibits fibroblast production of IL-6 but enhances its ability to stimulate acute phase responses.