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目的:探讨Ⅰ组亲代谢型谷氨酸受体(mGluR)配基对6-羟基多巴胺(6-OHDA)诱导的PC12细胞死亡及谷氨酸(Glu)释放的影响。方法:培养PC12细胞,以100μmol/LⅠ组mGluR激动剂(RS)-3,5-dihydroxyphenylglycine(DHPG)和拮抗剂DL-2-amino-3-phosphonopropionic acid(DL-AP3)预先剌激细胞1h,再加入6-OHDA100μmol/L共孵育24h,显微镜下观察细胞形态变化,用TUNEL法检测凋亡细胞,用噻唑蓝(MTT)法检测细胞存活率,并用高效液相色谱检测Glu的释放量。结果:6-OHDA 降低PC12细胞存活率(P<0.01),其诱导的Glu释放呈浓度和时间依赖性。Ⅰ组mGluR配基不能减少6-OHDA引起的PC12细胞死亡,也不影响6OHDA引起的Glu释放量。结论:Ⅰ组mGluR配基对6-OHDA引起死亡的PC12细胞无保护作用。
Objective: To investigate the effect of group Ⅰ metabotropic glutamate receptor (mGluR) ligand on 6-hydroxydopamine (6-OHDA) -induced PC12 cell death and glutamate (Glu) release. Methods: PC12 cells were cultured and pre-stimulated with mGluR agonist (RS) -3,5-dihydroxyphenylglycine (DHPG) and DL-2-amino-3-phosphonopropionic acid (DL-AP3) The cells were incubated with 100μmol / L 6-OHDA for 24 hours. The morphological changes were observed under a microscope. The apoptotic cells were detected by TUNEL assay. Cell viability was assayed by MTT assay and the release of Glu was determined by high performance liquid chromatography. Results: 6-OHDA decreased the survival rate of PC12 cells (P <0.01), and the release of Glu induced by it was concentration-dependent and time-dependent. Group I mGluR ligands can not reduce the PC-6 cell death caused by 6-OHDA, and also do not affect the amount of Glu released by 6OHDA. CONCLUSION: Group I mGluR ligand has no protective effect on PC12 cells caused by 6-OHDA.