Chondrogenic differentiation is a coordinated biological process orchestrated by various cell signaling pathways,involving complex pathways regulated at both transcriptional and posttranscriptional levels.Long noncoding RNAs (IncRNAs) are emerging as important regulators in the modulation of multiple cell processes.However,the potential roles of IncRNAs and their regu latory mechanisms in chondrogenic differentiation remain largely unclear.In this study,microarray was performed to detect the expression profiles of IncRNAs and messenger RNAs (mRNAs) during chondrogenic differentiation of murine chondrogenic cell line ATDC5.Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to explore their functions.Coding-noncoding co-expression (CNC) and competing endogenous RNA (ceRNA) networks were also constructed with bioinformatics methods.The results revealed that 1009 IncRNAs and 1206 mRNAs were differentially regulated during chondrogenic differentiation.GO and KEGG pathway analysis indicated that the principal functions of the transcripts were associated with system development and extracellular matrix-receptor interaction,TGF-β signaling,and PI3K-Akt signaling pathways.The CNC network showed that IncRNA AK136902 was positively correlated with prostaglandin F receptor (FP).The ceRNA network covered 3 IncRNAs,121 miRNA s and 241 edges.The upregulated IncRNA AK136902,AK016344,and ENSMUST00000180767 might promote chondrogenic differentiation by acting as ceRNAs.Knockdown of IncRNA A K136902 could inhibit the mRNA expression of FP and other chondrogenic related genes,including Aggrecan and Col2a1 during chondrogenic differentiation.Our results provide a new perspective on the modulation of IncRNAs during chondrogenic differentiation.