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目的运用随机扩增多态性DNA(RAPD)基因分型技术监测铜绿假单胞菌(PA)的医院感染情况,了解耐药表型与RAPD基因型关系,为确定院内交叉感染发生及院感控制提供依据。方法对10例ICU患者连续多次采样分离出的48株PA进行RAPD基因分型和药物敏感性试验。结果48株PA共分出9个基因型。6例患者为单一RAPD型PA菌株感染,4例患者检出2种RAPD型,有3组患者分别检出同型RAPD。耐药性最高的是环丙沙星(75%),其次是左氧氟沙星(73%),耐药性最低的为美洛培南(31%)。结论耐药表型与RAPD基因型之间不存在相关性;RAPD分型快速简单,对于流行病学调查确定院内交叉感染或流行具有重要意义。
Objective To detect the prevalence of nosocomial infection of Pseudomonas aeruginosa (PA) using random amplified polymorphic DNA (RAPD) genotyping and to understand the relationship between drug resistance phenotypes and RAPD genotypes. To determine the incidence of nosocomial infections and nosocomial infections Control provided basis. Methods RAPD genotyping and drug susceptibility testing were performed on 48 PA isolates from 10 consecutive ICU patients. Results 48 PA strains were divided into 9 genotypes. Six patients were infected with a single RAPD type PA strain, four were detected with two RAPD types, and three were detected with the same type of RAPD. The most resistant were ciprofloxacin (75%), followed by levofloxacin (73%) and meropenem (31%) with the lowest resistance. Conclusion There is no correlation between drug resistant phenotype and RAPD genotype. RAPD typing is rapid and simple, which is of great significance for epidemiological investigation to determine nosocomial infection or epidemic.