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目的探讨胚胎干细胞关键因子Nanog在人胶质瘤中的表达及意义。方法通过免疫荧光双标技术,分析40例胶质瘤组织内胚胎干细胞关键因子Nanog与肿瘤干细胞相关基因Nestin、CD133及胚胎干细胞全能性相关基因Oct4的共表达情况,并通过RT-PCR、Western blotting技术分析胶质瘤组织内Nanog与脑胶质瘤恶性程度之间的关系。结果 Nanog在胶质瘤组织中的表达随着胶质瘤病理级别增高而升高,而且超过50%的Nanog阳性细胞同时表达Nestin和CD133。95%以上的Nanog阳性细胞都同时表达胚胎干细胞全能性相关基因Oct4。结论胶质瘤组织中Nanog多表达在肿瘤干细胞中,与胶质瘤的恶性程度呈正相关,在胶质瘤的发生、发展过程中起着重要作用,为研究胶质瘤的起源及胶质瘤的诊断和预后判断提供帮助。
Objective To investigate the expression and significance of Nanog, a key factor of embryonic stem cells in human glioma. Methods The co-expression of Nanog, a key factor of embryonic stem cells in human glioma tissue, and Nestin, CD133 and Oct4 genes related to embryonic stem cells were analyzed by immunofluorescence double labeling technique. The expression of Oct4 was analyzed by RT-PCR and Western blotting The relationship between Nanog and the malignant degree of glioma in glioma tissues was analyzed. Results The expression of Nanog in glioma tissue increased with the pathological grade of glioma, and more than 50% of Nanog positive cells expressed both Nestin and CD13. More than 95.95% of Nanog positive cells expressed embryonic stem cell pluripotency Related Gene Oct4. Conclusion Nanog overexpression in glioma tissue is a positive correlation with the malignant degree of glioma in tumor stem cells and plays an important role in the genesis and development of glioma. In order to study the origin of glioma and the expression of glioma Diagnosis and prognosis to provide help.