论文部分内容阅读
目的:了解衢州市家禽、家畜肠出血性大肠埃希菌0157:H7带菌情况和菌型分布特征,以制定相应的防治对策。方法:6月份肠道传染病高发季节,采集动物粪便标本,对0157:H7菌株进行分离培养,并用PCR方法检测其毒力基因。结果:共采集动物粪便标本300份,检出O157:H7菌16株,总带菌率为5.33%,其中牛、羊、猪带菌率较高,分别为20.83%,12.70%和3.61%。经PCR检测,检出的所有菌株均携带SLT2与hly毒力因子,而SLT1阴性。结论:衢州市分离到带有毒力基因的肠出血性大肠埃希菌0157:H7,对人群健康构成了威胁,应加强O157:H7的综合监测。
Objective: To understand the characteristics of carriage and distribution of enterohemorrhagic Escherichia coli 0157: H7 in poultry and livestock in Quzhou, so as to formulate corresponding control measures. Methods: In the season of high incidence of intestinal infectious diseases in June, animal stool specimens were collected and the 0157: H7 strain was isolated and cultured, and its virulence genes were detected by PCR. RESULTS: A total of 300 samples of animal feces were collected and 16 strains of O157: H7 were detected with a total carrier rate of 5.33%. The rates of bovine, sheep and pig were 20.83%, 12.70% and 3.61% respectively. All the strains detected by PCR showed SLT2 and hly virulence factors, while SLT1 was negative. Conclusion: Enterohemorrhagic Escherichia coli 0157: H7 with virulence genes isolated from Quzhou City poses a threat to the health of the population. Therefore, comprehensive monitoring of O157: H7 should be strengthened.