论文部分内容阅读
本文提告了人扁桃体细胞在体外培养和诱生原发性抗SRBC反应的实验条件。发现比较合适的培养细胞密度是0.5～1.0×10~7个有核细胞/ml在添加混合的牛血清和马血清的培基中,比单独使用牛血清或马血清的培基能诱生出更多的抗体形成细胞(即空斑形成细胞PFC)。适当剂量的胰岛素、脂多糖或胸腺嘧啶核苷和次黄嘌呤都有增加PFC数的作用。人扁桃体细胞在体外培养中诱生出的抗SRBC PFC数一般是100～300个PFC/10~6个有核细胞。
This article reports the experimental conditions in which human tonsillar cells are cultured in vitro and induce primary anti-SRBC reactions. Found that more appropriate culture cell density is 0.5 ~ 1.0 × 10 ~ 7 nucleated cells / ml in the medium mixed with bovine serum and horse serum, compared with bovine serum alone or horse serum can induce more More antibodies form cells (ie plaque forming cells PFCs). Appropriate doses of insulin, lipopolysaccharide or thymidine and hypoxanthine have increased the role of PFC number. The number of anti-SRBC PFCs induced by in vitro cultured human tonsillar cells is typically 100-300 PFCs / 10-6 nucleated cells.