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[目的]检测胃癌患者肿瘤组织及其相应的癌旁组织和淋巴结组织中端粒酶逆转录酶(hTERT)基因启动子区域甲基化状态,并探讨其甲基化状态的改变与临床病理特征的关系。[方法]运用甲基化特异性PCR(MSP)方法,检测52例手术切除胃癌组织、癌旁组织及相关淋巴结中hTERT基因启动子区域甲基化状态,以同一标本正常组织作为阴性对照。[结果]正常胃黏膜组织未检测出hTERT表达,胃癌组织及癌旁组织、转移淋巴结中均检测出hTERT表达。转移淋巴结、胃癌组织中hTERT基因的甲基化阳性率分别为81.6%(31/38)、71.1%(37/52),明显高于癌旁组织的29.5%(13/52)(P<0.01)。胃癌组织hTERT基因甲基化阳性率与胃癌的临床分期、组织分化程度、肿瘤大小有相关性(P<0.05)。癌旁组织hTERT基因甲基化阳性率和胃癌的临床分期、肿瘤大小、组织分化程度、淋巴结转移具有相关性(P<0.05)。转移淋巴结hTERT基因甲基化阳性率则与临床及病理特征无关。[结论]胃癌组织及转移淋巴结中存在hTERT基因启动子区域的异常甲基化调控,可能参与了胃癌的发生与发展。
[Objective] To detect the methylation status of telomerase reverse transcriptase (hTERT) gene promoter region in gastric cancer tissues and its corresponding paracancerous tissues and lymph node tissues, and to investigate its methylation status and clinicopathological features Relationship. [Methods] Methylation-specific PCR (MSP) was used to detect the methylation status of hTERT promoter region in 52 cases of gastric cancer tissues, adjacent tissues and lymph nodes. The normal tissues of the same specimens were used as negative control. [Result] The expression of hTERT was not detected in normal gastric mucosa tissues, but hTERT expression was detected in gastric cancer tissues, paracancerous tissues and metastatic lymph nodes. The positive rates of hTERT gene methylation in metastatic lymph nodes were 81.6% (31/38) and 71.1% (37/52), respectively, which were significantly higher than those in paracancerous tissues (29.5%, 13/52, P <0.01) ). The positive rate of hTERT gene methylation in gastric cancer was correlated with clinical stage, histological differentiation and tumor size (P <0.05). The positive rate of hTERT gene methylation in paracancerous tissues was correlated with clinical stage, tumor size, histological grade and lymph node metastasis (P <0.05). The positive rate of hTERT gene methylation in lymph node metastasis was not related to clinical and pathological features. [Conclusion] The aberrant methylation of hTERT promoter region in gastric cancer tissues and metastatic lymph nodes may be involved in the occurrence and development of gastric cancer.