利用聚乙二醇(PEG)处理对植物原生质体的直接转化需要一个逐步缓慢的PEG溶解过程。PEG溶解所需要的时间限制了能够完成和比较的试验次数。最近,Purdue大学的科学家L、A、Lyznik和同事描述了植物原生质PEG介导转化的一种快速而简便的改良方法。这种方法包括原生质体、DNA和PEG混合物的转移,PEG直接放置在琼脂糖块上,而不是溶解PEG。PEG靠琼脂糖中存在的液体原生质培养基来溶解。 Direct conversion of plant protoplasts using polyethylene glycol (PEG) treatment required a gradual slow PEG dissolution process. The time required for PEG dissolution limits the number of tests that can be done and compared. Recently, scientists at Purdue University, L, A, Lyznik, and colleagues described a quick and easy way to improve plant-mediated PEG-mediated transformation. This method involves the transfer of protoplasts, DNA and PEG mixes, and the PEG is placed directly on agarose pellets instead of dissolving PEG. PEG dissolves in liquid protogenic medium present in agarose.