1引言组织蛋白酶B(CB)是溶酶体内半胱氨酸蛋白水解酶,属于木瓜蛋白酶家族,其特异性底物为Z-Arg-Arg-7-氨基-4-甲基香豆素[1],CB催化底物肽键断裂生成7-氨基-4-甲基香豆素。近年来的研究表明,CB在肿瘤的发展中起作用的一类关键酶[2],CB通过多种机制促进血管形成,从而促进肿瘤生长,同时降解基底膜主要成分以及激活胶原酶、尿激酶等其它蛋白酶发生协同作用,使肿瘤易侵袭、转移和扩散。在肿瘤组织中还发现CB与CB抑制剂平衡作用也参与肿瘤的发 1 Introduction Cathepsin B (CB) is a lysosomal cysteine ​​protease belonging to the papain family with a specific substrate of Z-Arg-Arg-7-amino-4-methylcoumarin [1 ], CB catalyzed peptide bond cleavage to generate 7-amino-4-methylcoumarin. In recent years, studies have shown that CB plays a key role in the development of tumors [2], CB through a variety of mechanisms to promote blood vessel formation, thereby promoting tumor growth, while degrading the main components of the basement membrane and activation of collagenase, urokinase Other proteases and other synergistic effect, so that tumor invasion, metastasis and proliferation. It is also found in tumor tissues that the balance of CB and CB inhibitors is also involved in tumor development