论文部分内容阅读
报道用生物发光技术(BL法)取代现行卡介苗菌落形成单位计数法(CFU)的实验研究。建立了标准化BL检测方法:并成功地设计了抽提效果良好的超声-氯仿卡介苗ATP提取法。ATP标准品含量与ATPBL值具有明显剂量依赖关系;卡菌苗菌液和冻干卡介苗菌液的BL值与各相应菌液的CFU数密切相关,相关系数分别为0.8155和0.8484(p<0.05);卡介苗ATPBL值批间变异系数(3.2～4.4％)明显低于CFU数的批间变异系数(10.4～11.2％)。
Reported the use of bioluminescence technology (BL method) to replace the current BCG colony forming unit counting method (CFU) experimental study. The standard BL detection method was established and the sonication of chloroform-BCM ATP extraction was successfully designed. ATP content and ATPBL value has a significant dose-dependent relationship; Bacillus subtilis bacteria and freeze-dried BCG bacterial broth value and the corresponding bacterial CFU number is closely related to the correlation coefficients were 0.8155 and 0.8484 (p <0.05) ; The coefficient of variation (BCG) of BCG ATPBL was significantly lower than that of CFU (between 10.4% and 11.2%).