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本实验采用平板计数和末端限制性片段长度多态性技术(T-RFLP),分析了有机碳源添加对凡纳滨对虾养殖系统中人工基质(筛绢网)表面生物膜菌群结构组成及其动态变化的影响。研究表明,当人工基质表面菌膜成熟时,实验组人工基质表面异养菌数量高于对照组,但没有显著性差异;弧菌数及弧菌占总异养菌的比例分别极显著低于对照组91.85%和96.98%(p<0.01)。T-RFLP图谱分析表明,两处理组中126、432、434、436、485、487和488 bp等T-RF相对丰度变化显著;其中,29 d时实验组菌群种类丰富度指数和Shannon-Weiner指数分别高于对照组100%和33.9%。移动窗口分析表明,对照组菌群结构变化率△t为23.2%±26.38%,高于实验组的12.4%±0.3%。综上可得,有机碳驱动不仅能够促进异养菌的生长,竞争性抑制弧菌的增殖,而且可以提高养殖池塘中人工基质表面菌群结构的稳定性。
In this study, the plate count and terminal restriction fragment length polymorphism (T-RFLP) were used to analyze the biofilm composition of the artificial substrate (silk screen mesh) biofilm in the Litopenaeus vannamei culture system with organic carbon source The impact of its dynamic changes. The results showed that the number of heterotrophic bacteria on the surface of the artificial substrate was higher than that of the control group, but no significant difference was found when the surface of the artificial substrate was mature. The numbers of Vibrio and Vibrio bacteria were significantly lower than that of the total heterotrophic bacteria The control group 91.85% and 96.98% (p <0.01). T-RFLP analysis showed that the relative abundance of T-RFs such as 126,432,434,436,485,487 and 488 bp in the two treatment groups changed significantly. Among them, the species richness index and Shannon Weiner index were higher than the control group 100% and 33.9%. The mobile window analysis showed that the change rate of bacterial flora in the control group was 23.2% ± 26.38%, which was higher than 12.4% ± 0.3% of the experimental group. In summary, organic carbon drive can not only promote the growth of heterotrophic bacteria, competitive inhibition of Vibrio proliferation, but also can improve the stability of the artificial substrate surface microbial flora structure.