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目的探讨大肠癌及淋巴结转移中血管内皮生长因子(VEGF)A、C的表达特点及其与临床病理特征的关系。方法应用链霉抗生物素蛋白过氧化酶染色方法,检测12例正常大肠黏膜、18例大肠腺瘤、63例大肠腺癌原发灶以及19例淋巴结转移灶VEGFA、C的表达状况。结果VEGFA、C在正常大肠黏膜中无阳性表达,在大肠腺瘤有少量表达,大肠腺癌原发灶中VEGFA、C阳性表达率分别为57.14%、55.56%,明显高于正常组织和腺瘤(p<0.05);19例大肠癌原发灶和淋巴结转移灶中VEGFC高表达阳性率分别为42.11%(8/19)、73.68%(14/19),两者差异有统计学意义(P<0.05)。结论VEGFA、C的过度表达与大肠腺瘤恶变及转移有密切关系,VEGFC突出地与区域淋巴结转移明显相关。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) A and C in colorectal carcinoma and lymph node metastasis and its relationship with clinicopathological features. Methods The expression of VEGFA and C in 12 cases of normal colorectal mucosa, 18 cases of colorectal adenoma, 63 cases of colorectal adenocarcinoma and 19 cases of lymph node metastasis were detected by streptavidin peroxidase staining method. Results There was no positive expression of VEGFA and C in normal colorectal mucosa and little expression in colorectal adenoma. The positive expression rates of VEGFA and C in primary colorectal adenocarcinoma were 57.14% and 55.56%, respectively, which were significantly higher than those in normal tissues and adenomas (p <0.05). The positive expression rates of VEGFC in 19 cases of primary colorectal cancer and lymph node metastases were 42.11% (8/19) and 73.68% (14/19) respectively, the difference was statistically significant (P <0.05). Conclusion The overexpression of VEGFA and C is closely related to the malignant transformation and metastasis of colorectal adenoma. VEGFC is significantly associated with regional lymph node metastasis.