论文部分内容阅读
农业土壤一旦被粪便性病菌污染,将给人类带来潜在的感染病菌的风险。通过选择大肠杆菌作为粪便性病菌的模式菌接种于土壤中,在温室条件下采用日晒热消毒法对大肠杆菌进行灭菌试验,探讨日晒消毒法的生物安全控制效果。结果表明,日晒处理使土壤温度在1周内升至40℃,并在大部分处理时期维持在40～46℃;处理区土壤中的大肠杆菌数在1周内从105cfu/g干土降至检测限以下(<0.08cfu/g干土),而对照区土壤中的大肠杆菌数在4周后仍可被检测出。由此可知,温室土壤在因施用未腐熟堆肥或动物粪便而被病菌污染时,日晒消毒是快速有效的生物安全控制手段。
Once contaminated by stool pathogens, agricultural soils will pose a potential risk of infection to humans. Escherichia coli was selected as the mode bacteria of stool pathogenic bacteria inoculated into the soil, and the bacterium was sterilized by the method of sun-drying and hot disinfection under the greenhouse conditions to study the biosafety control effect of the sun-drying disinfection method. The results showed that the soil temperature increased to 40 ℃ within 1 week and maintained at 40 ~ 46 ℃ in most of the treatment period. The number of E. coli in the soil of the treatment area decreased from 105cfu / g dry soil within 1 week To below the detection limit (<0.08 cfu / g dry soil), whereas the number of E. coli in the soil in the control area was still detectable after 4 weeks. From this we can see that when the greenhouse soil is contaminated by germs due to the application of non-compost compost or animal excrement, insolation disinfection is a fast and effective biological safety control measure.