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目的：探讨血吸虫肠相关循环抗原ＣＡＡ与ＣＣＡ诊断靶微粒特异性的差别，并试探获取其纯化制品用于定量检测的标准系列。方法：对两组分进行了亲和层析及阴离子交换剂高效液相纯化分离，并应用单抗检测系统进行同相和异相交互测试。结果：Ｍｏｎｏ－Ｑ－ＨＰＬＣ（高效液相层析）梯度洗脱分离ＡＷＡｊ－ＴＣＡ可溶组分，可获得一个带阳离子活性的非结合ＣＣＡ－１洗脱峰及３个大小不等的、带阳离子活性的ＣＣＡ－２、ＣＣＡ－３及ＣＣＡ－４非结合洗脱峰，以及一个带阴离子活性ＣＡＡ－１洗脱峰。ＣＡＡ活性峰在峰谱上与ＣＣＡ－３有部分重叠。与单抗亲和层析纯品的活性对比测定显示ＣＣＡ－１与ＣＣＡ－２为该组分的主要构成，但ＣＣＡ－２及ＣＡＡ－１在本实验条件下均有微量的相互杂染。同相和异相双位点ＥＬＩＳＡ的４种组合交互检测，展示ＣＣＡ只能在捕获与检测抗体同为抗ＣＣＡ单抗的一种组合中被检出，而另３种组合只能测出ＣＡＡ组分。结论：血吸虫肠相关ＣＣＡ组分为一兼含两性电荷的分子混合体；而ＣＡＡ分子上具有一个可被抗ＣＣＡ单抗识别的活性表位位点，从而可能影响纯化分离和特异检测。
OBJECTIVE: To investigate the specificity of the target particles for the diagnosis of schistosoma intestinal associated circulating antigen CAA and CCA, and to explore the standard series of the purified products for quantitative detection. Methods: The two fractions were purified by affinity chromatography and anion exchange liquid high performance liquid chromatography (HPLC). The single phase detection system was used to carry out in-phase and out-of-phase interaction tests. Results: Mono-Q-HPLC (High Performance Liquid Chromatography) gradient elution was used to separate the AWAj-TCA soluble fraction. A non-binding CCA-1 eluting peak with cationic activity and three bands Cationic CCA-2, CCA-3 and CCA-4 non-binding elution peaks, and an anion-active CAA-1 elution peak. The peak of CAA activity partially overlaps with CCA-3 in the peak spectrum. The results of CCA-1 and CCA-2 assay showed that CCA-2 and CAA-1 were slightly mixed with each other in this experiment. The four combinations of in-phase and out-of-phase two-site ELISA were interactively detected, showing that CCA was detected only in a combination of capture and detection antibodies with anti-CCA mAb, whereas the other three combinations detected only CAA Minute. CONCLUSIONS: The schistosomal enterocolitica CCA fraction is a mixture of molecules containing both amphoteric charges. However, the CAA molecule has an active epitope that can be recognized by anti-CCA monoclonal antibody, which may affect purification and specificity.