论文部分内容阅读
REMAP是基于反转录转座子的一种分子标记技术,具有检测灵敏和多态性高的优点。本研究首次将REMAP技术应用于琼枝(Betaphycus gelatinae)、长心卡帕藻(Kappaphycus alvarezii)和麒麟菜(Eucheuma denticulatum)红翎菜科海藻的群体变异研究中。本研究共筛选出20对具有多态性的引物,在24株无性繁殖海藻中扩增得到500个位点,平均每对引物扩增位点数为25。研究结果表明,该标记技术在红翎菜科海藻中具有很好的适用性和通用性。红翎菜科海藻五个群体之间多态位点比例为100%,群体内部多态位点比例为3.8%~8.8%,同一藻株不同组织部位之间多态位点比例为18.62%~39.54%,证实了红翎菜科海藻在营养生长中广泛存着较高程度的变异,这为红翎菜科海藻种质资源创新及其良种选育提供了一种新的标记技术。
REMAP is a molecular marker technique based on retrotransposons with the advantage of high detection sensitivity and high polymorphism. This study, for the first time, applied REMAP technology to the population variation study of Betaphycus gelatinae, Kappaphycus alvarezii and Eucheuma denticulatum. In this study, 20 pairs of polymorphic primers were screened and 500 loci were amplified in 24 clones of asexual seaweed, with an average of 25 loci per primer pair. The results show that the labeling technique has good applicability and versatility in the red seaweed seaweed. The percentage of polymorphic loci was 100% in five populations of Leymus chinensis, and the percentage of polymorphic loci in populations was 3.8% -8.8%. The percentage of polymorphic loci in different tissues of the same strain was 18.62% 39.54%. It is confirmed that there is a wide range of variation in the vegetative growth of the seaweed, which provides a new marker technology for the innovation of red sea cucumber seaweed germplasm resources and their breeding.