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目的:观察加参方对大鼠心肌梗死后早期心室重构及炎性反应的影响。方法:采用结扎大鼠左冠状动脉前降支的方法建立大鼠心肌梗死模型,将术后24h存活大鼠随机分为5组:假手术组、模型组、加参方3g组、加参方6g组和氯沙坦组。药物干预4周后超声测量心脏结构及功能,利用Masson染色和羟脯氨酸测定法观察胶原蛋白的分布及含量,免疫组化法检测单核/巨噬细胞,并采用Western blot法确定细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)的蛋白表达量,ELISA法测定肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)和单核细胞趋化蛋白-1(MCP-1)的含量。结果:与模型组比较,加参方6g剂量能够明显改善心脏功能,降低缺血危险区心肌组织中胶原蛋白含量,减少单核/巨噬细胞浸润及炎性因子的表达量(P<0.05),该作用与氯沙坦相似。结论:加参方具有改善心肌梗死早期心室重构的作用,其机制可能与抑制炎性反应有关。
Objective: To observe the effect of Jia Shen Fang on early ventricular remodeling and inflammatory reaction after myocardial infarction in rats. Methods: A rat model of myocardial infarction was established by ligation of the left anterior descending coronary artery in rats. The rats were randomly divided into 5 groups: sham operation group, model group, ginseng group 3g group, 6g group and losartan group. The cardiac structure and function were measured by ultrasound four weeks after drug intervention. The distribution and content of collagen were observed by Masson staining and hydroxyproline assay. The expression of monocyte / macrophage was detected by immunohistochemistry. (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were detected by enzyme-linked immunosorbent assay (ELISA). The levels of TNF-α, IL- The content of nuclear chemotactic protein-1 (MCP-1). Results: Compared with the model group, adding 6g dose of Shenfang could significantly improve cardiac function, decrease the content of collagen and decrease the infiltration of monocytes / macrophages and the expression of inflammatory cytokines (P <0.05) , The effect is similar to losartan. Conclusion: Ganshenfang can improve the early ventricular remodeling after myocardial infarction. Its mechanism may be related to inhibiting the inflammatory reaction.