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[目的]对农杆菌介导转化获得的稻瘟病抗性基因Pi-d2的水稻植株稳定后代的主要农艺性状和米质进行了分析,以期为转基因水稻的生态学和安全性评价提供的依据。[方法]在田间调查了转Pi-d2基因水稻植株的主要农艺性状,并对转基因水稻稻米理化特性和氨基酸含量进行测定。[结果]外源基因Pi-d2的导入可引起受体植株后代农艺性状可遗传的变异。大部分转基因植株生长正常,整齐一致,与对照相比,一半左右植株株高变高或矮。分蘖数、穗长、有效穗、结实率和千粒重与对照无明显差异,少部分表现增加或减少的趋势。稻米的氨基酸测定结果表明转抗病基因水稻改变了原亲本的总氨基酸含量,呈下降趋势,但淀粉含量、胶稠度和糊化温度并未改变。[结论]转Pi-d2基因入水稻中对植株株高影响明显,但对稻米的理化特性影响不大。
[Objective] The research aimed to analyze the main agronomic traits and quality of stable offspring of rice plant Pi-d2, which is obtained from Agrobacterium tumefaciens mediated transformation, in order to provide the basis for the ecological and safety evaluation of transgenic rice. [Method] The main agronomic characters of Pi-d2 transgenic rice plants were investigated in the field. The physicochemical properties and amino acid content of transgenic rice were determined. [Result] The introduction of exogenous gene Pi-d2 caused the genetic variation of agronomic traits of the recipient plants. Most of the transgenic plants grow normally and neatly, and about half of the plant height is higher or shorter than that of the control. The number of tillers, spike length, effective panicle, seed setting rate and 1000-grain weight had no significant difference with the control, but some showed the tendency of increasing or decreasing. The results of amino acid analysis of rice showed that the resistance to transgenic rice changed the total amino acid content of the original parent, showing a downward trend, but the starch content, gel consistency and gelatinization temperature did not change. [Conclusion] Transgenic Pi-d2 gene had a significant effect on plant height in rice, but had little effect on the physicochemical properties of rice.