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目的观察常见实验动物离体食管固定后的收缩变化规律,为动物食管的组织学研究奠定基础。方法测量各实验动物食管离体后长度,并将离体后的动物食管分为2组,分别用体积分数10%的甲醛和体积分数90%的乙醇固定48 h再测量固定后的长度,比较收缩率。结果各实验动物食管固定后长度均发生了收缩,同一种动物食管固定前后长度比较差别有统计学意义(P<0.05);但2组用甲醛与乙醇固定后的收缩率比较差别无统计学意义(P>0.05)。结论甲醛和乙醇这2种化学固定方法均能影响动物食管固定后的收缩变化,但这2种固定方法对食管固定引起的收缩变化无明显差别。
Objective To observe the changes of contractility of common esophageal mucosa after esophagectomy in vitro, and to lay the foundation for the histological study of esophagus in animals. Methods The esophageal length of each experimental animal was measured. The esophagus was divided into two groups. The fixed esophagus were fixed with 10% formaldehyde and 90% ethanol for 48 h, respectively. Shrinkage. Results The lengths of esophageal fixed in experimental animals all shrank. The length of esophagus fixed in the same kind of animals was significantly different (P <0.05). However, there was no significant difference in the contractile rate between two groups after fixed with formaldehyde and ethanol (P> 0.05). Conclusions Formaldehyde and ethanol, the two chemical fixation methods, can all affect the contractile changes after esophageal fixation in animals. However, there is no significant difference between the two fixation methods on the contraction induced by esophageal fixation.