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目的评价PCR法、ELISA法和试条法检测我国婴儿利什曼原虫无症状感染犬的潜能。方法用两组PCR引物RV1-RV2和K13A-K13B检测动物源型黑热病疫区健康犬静脉血和骨髓中利什曼原虫特异DNA,以利什曼原虫可溶性抗原为包被抗原的ELISA法和rk39-dipstick试条法分别检测利什曼原虫特异抗体,并比较各种检测方法的敏感性差异。结果PCR法检测抗凝静脉血和骨髓的阳性率分别为50.63%(40/79)和69.62%(55/79),两种样本总检出率为77.21%(61/79);ELISA法检测的阳性率为22.22%(16/72),而rk39-dipstick试条检测的阳性率为33.33%(19/57)。结论我国动物源性黑热病疫区利什曼原虫无症状感染犬的比例相当高,以骨髓为样本的PCR检测法为较精确的犬无症状感染检测方法。
Objective To evaluate the potential of asymptomatic infection of Leishmania infantum in dogs by PCR, ELISA and strip method. Methods Two pairs of PCR primers RV1-RV2 and K13A-K13B were used to detect Leishmania specific DNA in healthy dog’s venous blood and bone marrow of zoonotic disease-free zoonotic area. ELISA was performed using Leishmania soluble antigen as antigen and rk39 The leishmania-specific antibodies were detected by the -dipstick method, and the sensitivity of different detection methods was compared. Results The positive rates of anticoagulation venous blood and bone marrow by PCR were 50.63% (40/79) and 69.62% (55/79), respectively. The total detection rate of the two samples was 77.21% (61/79) Positive rate was 22.22% (16/72), while the positive rate of rk39-dipstick test strip was 33.33% (19/57). Conclusion Asymptomatic infection of Leishmania in animal origin of SARS in China is very high. PCR detection method using bone marrow as a sample is a more accurate detection method for asymptomatic infection in dogs.