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目的:探索巴氯芬对大鼠初级感觉神经元膜NMDA激活电流的调制作用。方法:全细胞膜片箝技术在新鲜分离的背根神经节(DRG)细胞上进行实验。结果:DRG细胞外加巴氯芬(1-100μmol·L~(-1))未记录到可检测的膜电流改变,但预加巴氯芬对NMDA激活电流则有明显的抑制作用,巴氯芬100μmol·L~(-1)对NMDA(100 μmol·L~(-1))激活电流的抑制可达52％±14％(n=11,P<0.01),此抑制作用被GABA_B受体的拮抗剂沙氯芬(100 μmol·L~(-1))所取消,结论:初级感觉神经元膜上GABA_B受体的激活对NMDA受体介导的膜电流具有抑制作用。
AIM: To investigate the modulation of baclofen on the NMDA activation current in rat primary sensory neurons. METHODS: Whole-cell patch-clamp techniques were performed on freshly isolated DRG cells. RESULTS: No detectable change of membrane current was recorded in DRG cells plus baclofen (1-100 μmol·L -1), but pretreatment with baclofen significantly inhibited NMDA activation. Baclofen The inhibitory effect of 100μmol·L -1 on the activation of NMDA (100 μmol·L -1) was 52% ± 14% (n = 11, P <0.01). The inhibitory effect was inhibited by GABA_B receptor (100 μmol·L -1) antagonist. Conclusion: The activation of GABA_B receptor on the primary sensory neuron membrane can inhibit the NMDA receptor-mediated membrane current.