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本文报道地里纤恙螨雄虫人工接种恙虫病立克次体后与雌虫配对培养和传代，应用幼虫叮咬小鼠分离立克次体和PCR结合核酸杂交（核酸杂交检测PCR产物）检测子代体内立克次体的结果。幼虫叮咬小鼠分离立克农作检查第3子代幼虫体内立克次体阳性。PCR结合核酸杂交检测佛山市的病人恙虫病立克次休（未定株）接种雄虫和健康雌虫配对的第1、2、3子代成虫体内立克次体DNA阳性。Karp株接种雄虫的第1子代幼虫体内立克次体的幼虫叮咬小鼠法和PCR结合核酸杂交均呈阳性。
In this paper, the male chigger mites were inoculated with Rickettsia tsutsugamushi Rickettsia rickettsi and mated to the females, and the larvae were stained by the mice to separate the rickettsia and the PCR-combined nucleic acid hybridization (PCR) The results of the in vivo rickettsia. Larvae bites mice isolated Rick Farms to examine third-generation larvae in vivo rickettsial positive. PCR combined with nucleic acid hybridization detection of patients with Foshan tsutsugamushi Rickettsu (undetermined) inoculated male and healthy female paired first and second-generation offspring of adult rickettsial DNA positive. The larval bite mice of the first generation larvae of the Karp strain inoculated with the male were both positive for PCR and nucleic acid hybridization.