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目的:探讨大承气颗粒对大鼠不完全性肠梗阻小肠上皮细胞的保护作用及机理。方法:采用贯穿缝扎末端回肠肠管的方法建立大鼠不完全性小肠梗阻模型,观察肠梗阻72h的小肠组织形态学、二胺氧化酶(DAO)活性、肠上皮细胞凋亡情况、小肠黏膜上皮细胞内Ca2+水平及血浆与小肠组织NO、MDA、SOD的变化。结果:模型组小肠黏膜损伤评分增加,血浆DAO活性明显升高,小肠组织DAO活性降低,肠组织细胞染色体DNA电泳可见典型的DNA梯状带生成,且小肠上皮细胞凋亡指数明显增加,肠上皮细胞内Ca2+浓度以及NO、MDA水平升高,SOD水平降低;大承气颗粒给药组不同程度地降低小肠黏膜损伤评分,降低血浆DAO及升高小肠组织DAO活性,降低小肠上皮细胞凋亡指数,且电泳呈现减弱的DNA梯状图谱、小肠黏膜上皮细胞内Ca2+浓度以及NO、MDA水平降低和SOD水平升高。结论:大承气颗粒能改善不完全性肠梗阻小肠组织形态学,阻遏肠黏膜上层绒毛中DAO的受损,抑制肠梗阻时的肠上皮细胞凋亡的过度增加,保护不完全性肠梗阻肠黏膜结构的完整性,具有肠屏障保护功能,上述作用机制可能与抗自由基及降低小肠黏膜上皮细胞内钙离子超载,从而减轻小肠黏膜上皮细胞损伤等有关。
Objective: To investigate the protective effect and mechanism of Dachengqi granule on intestinal epithelial cells of incomplete intestinal obstruction in rats. Methods: The model of incomplete intestinal obstruction was established by suturing the distal ileum intestinal tract. The intestinal histomorphology, diamine oxidase (DAO) activity, intestinal epithelial cell apoptosis, intestinal mucosal epithelium Intracellular Ca2 + level and the changes of NO, MDA and SOD in plasma and small intestine. Results: The intestinal mucosal lesion score of model group increased, the activity of DAO in plasma increased obviously, the activity of DAO in small intestine decreased. The typical DNA ladder formed in chromosome DNA of intestine tissue, and the apoptotic index of intestinal epithelium increased obviously. Intracellular Ca2 + concentration and NO, MDA levels increased, SOD levels decreased; Dachengqi granule administration group to different degrees reduce the intestinal mucosal injury score, reduce plasma DAO and increased intestinal activity of DAO, reduce the intestinal epithelial cell apoptosis index , And electrophoresis showed weakened DNA ladder, intestinal mucosal epithelial cells Ca2 + concentration and NO, MDA levels decreased and SOD increased. Conclusion: Dachengqi granule can improve intestinal histomorphology of incomplete intestinal obstruction, prevent dao dao dao dao damage in intestinal mucosa, inhibit the excessive increase of intestinal epithelial apoptosis in intestinal obstruction, protect incomplete intestinal ileus The integrity of the mucosal structure, with intestinal barrier function, the mechanism may be related to anti-free radicals and reduce intestinal epithelial cells in the calcium overload, thereby reducing intestinal mucosal epithelial cell damage and so on.