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目的研究原癌基因c-erbB2和肺表皮生长因子受体(EGFR)的表达与大鼠肺纤维化的相关性,探讨肺纤维化的发生机制。方法采用博莱霉素气管注射法制造鼠肺纤维化模型。30只Wistar大鼠随机分为模型组和对照组,分别于第1、14、28天处死大鼠5只并留取标本,采用免疫组化SP法检测在不同程度肺纤维化条件下c-erbB2和肺内源性EGFR的表达,分析其与肺纤维化的相关性。结果①模型组c-erbB2和EGFR表达在各时间点均与对照组有统计学差异(P<0.01);②模型组c-erbB2表达1d最显著,EGFR表达28d达到顶峰,与同组其他时间点均有统计学差异(P<0.01);③模型组c-erbB2和EGFR水平在第14天与最低水平组(分别为第28天和第1天)比较有统计学差异(P<0.01);④模型组c-erbB2和EGFR表达与肺纤维化程度密切相关(相关系数r分别为-0.835和0.77,P<0.01)。结论 c-erbB2活化的同时,EGFR表达也逐渐增强,且c-erbB2、EGFR的活化水平与肺纤维化程度密切相关;c-erbB2和EGFR激活可能参与了肺纤维化的发生。
Objective To study the relationship between the expression of proto-oncogene c-erbB2 and epidermal growth factor receptor (EGFR) and pulmonary fibrosis in rats and to explore the mechanism of pulmonary fibrosis. Methods Bleomycin-induced tracheal injection of lung fibrosis model. Thirty Wistar rats were randomly divided into model group and control group. Five rats were killed on the 1st, 14th and 28th day, respectively. The specimens were collected for immunohistochemical staining for c- erbB2 and lung endogenous EGFR expression analysis of its correlation with pulmonary fibrosis. Results ① The expressions of c-erbB2 and EGFR in the model group were significantly different from those in the control group at each time point (P <0.01). ② The expression of c-erbB2 in the model group was the most significant at 1 day. The expression of EGFR peaked at 28 days, (P <0.01). ③The levels of c-erbB2 and EGFR in the model group were statistically different from those in the lowest level on day 14 (day 28 and day 1, respectively) (P <0.01) ; ④The expression of c-erbB2 and EGFR in model group was closely related to the degree of pulmonary fibrosis (r = -0.835 and 0.77 respectively, P <0.01). Conclusions The activation of c-erbB2 is accompanied with the gradual increase of EGFR expression. The activation of c-erbB2 and EGFR is closely related to the degree of pulmonary fibrosis. The activation of c-erbB2 and EGFR may be involved in the pathogenesis of pulmonary fibrosis.