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目的 探讨血红素加氧酶 一氧化碳 (HO CO)体系对缺氧性肺动脉高压幼年大鼠肺动脉超微结构的影响。 方法 将 2 6只Wistar大鼠随机分为 4组 :对照组、低氧组、低氧 +锌原卟啉 (ZnPP)组和低氧 +一氧化碳(CO)组。以右心导管法测定肺动脉压力 ,并对大鼠肺动脉进行超微结构观察。 结果 低氧组大鼠肺动脉超微结构发生明显改变 :内皮细胞呈柱状 ,部分核突入管腔 ,排列呈栅状 ,内皮细胞肿胀 ,胞浆内可见大量空泡 ,线粒体肿胀 ,内质网扩张 ;平滑肌细胞胞体肥大 ,胞浆中肌丝和致密斑减少 ,线粒体、粗面内质网和游离核糖体等细胞器增多。约半数平滑肌细胞处于收缩表型 ,余呈过渡表型或合成表型 ,有肺血管结构重构形成。低氧 +ZnPP组肺血管结构重建程度较低氧组加重 :内皮细胞呈柱状 ,呈栅状排列 ,部分脱落致管腔 ,胞浆内可见大量空泡 ,线粒体肿胀 ,内质网扩张 ;平滑肌细胞形态不规则 ,胞体肥大 ,胞浆中肌丝和致密斑明显减少 ,粗面内质网和游离核糖体等细胞器明显增多。低氧组 +CO组肺血管结构重建程度较低氧组减轻 :内皮细胞胞体扁平 ,内衬血管内壁 ,其肿胀程度较低氧组减轻 ,空泡明显减少 ;平滑肌细胞改变较低氧组为轻 ,约半数平滑肌细胞处于收缩表型 ,余呈合成表型或过渡表型。 结论 HO CO系统对缺氧性肺?
Objective To investigate the effect of heme oxygenase (CO) on the ultrastructure of pulmonary artery in hypoxic pulmonary hypertensive rats. Methods Twenty - six Wistar rats were randomly divided into 4 groups: control group, hypoxia group, hypoxia + ZnPP group and hypoxia + CO group. Pulmonary artery pressure was measured by right heart catheterization and the ultrastructure of pulmonary artery in rats was observed. Results The ultrastructure of pulmonary artery in hypoxia rats changed obviously. The endothelial cells were columnar, some of them protruded into the lumen, arranged in a grid, the endothelial cells were swollen, a large number of vacuoles were found in cytoplasm, the mitochondria were swollen and the endoplasmic reticulum was dilated. Smooth muscle cell body hypertrophy, filamentous cytoplasm and diminished plaque, mitochondria, rough endoplasmic reticulum and free organelles and other organelles increased. About half of smooth muscle cells in the contraction phenotype, Yu transitional phenotype or synthetic phenotype, pulmonary vascular remodeling. Hypoxia + ZnPP group pulmonary vascular structural remodeling less oxygen group increased: endothelial cells were columnar, arranged in a grid, part of the shedding of the lumen, a large number of vacuoles visible in the cytoplasm, mitochondria swelling, endoplasmic reticulum expansion; smooth muscle cells Irregular shape, hypertrophy of cell body, obvious decrease of myofilament and dense plaque in cytoplasm, increase of organelles such as rough endoplasmic reticulum and free ribosome. In the hypoxia group and CO group, the remodeling of the pulmonary vascular structure was less than that in the hypoxia group. The endothelial cells were flat and lined with blood vessel inner wall. The extent of swelling was lower than that in the hypoxia group and the vacuoles were significantly reduced. The changes of the smooth muscle cells in the hypoxia group were lighter , About half of the smooth muscle cells in the contraction phenotype, Yu synthetic phenotype or transitional phenotype. Conclusion HO CO system on hypoxic lung?