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目的 本文旨在研究三苯氧胺在膀胱癌T -2 4细胞株中可能有的化疗增敏作用。材料与方法 应用MTT方法 ,以膀胱肿瘤T-2 4细胞株为研究对象 ,比较单独应用化疗药物 (ADM、MMC、Thiotepa、VCR)与化疗药物合并应用三苯氧胺 (Tamoxifen 5 μM ,10 μM )对细胞生长抑制率效应 ,通过化疗药物的量—效关系曲线 ,观察三苯氧胺是否有特定的化疗增敏作用。结果 三苯氧胺在浓度为 5μM、10 μM时 ,能分别使ADM和VCR的IC50 下降 1 95、7 0 6倍和 6 94、14 5 5倍 ,差异有显著性 (P <0 0 5 ) ;而三苯氧胺在 5 μM、10 μM的浓度下 ,对MMC、Thiotepa的IC50 的影响 ,无统计学意义。结论 在体外试验中 ,三苯氧胺在 5 μM和 10 μM的浓度下能显著性地促进膀胱肿瘤T -2 4细胞株对ADM和VCR的化疗敏感性
Objective This study aimed to investigate the possible chemosensitization of tamoxifen in bladder cancer T-24 cell line. MATERIALS AND METHODS MTT assay was used to investigate the effects of Tamoxifen (5 μM, 10 μM) combined with chemotherapeutic agents (ADM, MMC, Thiotepa, VCR) alone on bladder cancer T-2 4 cell lines. Growth inhibition rate effect, through the dose-effect relationship curve of chemotherapy drugs to observe the tamoxifen has a specific chemosensitizing effect. Results Tamoxifen decreased the IC50 of ADM and VCR by 95%, 70% and 694, 1455 times, respectively, at a concentration of 5 μM and 10 μM, the difference was significant (P <0 05); while tamoxifen At concentrations of 5 μM and 10 μM, the IC50 for MMC and Thiotepa was not statistically significant. Conclusion In vitro, tamoxifen significantly promoted chemosensitivity to ADM and VCR in bladder tumor T-24 cell lines at concentrations of 5 μM and 10 μM