本文作者应用Eagle改良的最小基本培养基MEM,内含5%胎牛血清,葡萄糖含量为RPMI 1640培养基的5倍,所用的气体含10%CO_2。针对约氏疟原虫偏爱侵入网织红细胞,因此在培养中使用两种来源的网织红细胞。一种是苯肼(Phenylhydrazine)诱发者:即未感染的CBA小鼠自腹腔内给予盐酸苯肼2.5mg,8天后,小鼠血内即含有20～30%的网织红细胞;另一种称之为“危象后期”(post-crisis)者:即小鼠接种感染后,用药物清除疟原虫,血内即含有40～60%未感染的网织红细胞。培养在37℃和20℃两种温度下进行。 The authors used MEM modified Eagle minimal basic medium containing 5% fetal bovine serum, glucose content of 5 times the RPMI 1640 medium, the gas used in 10% CO_2. Plasmodium yoelii prefers invasion of reticulocytes, so two sources of reticulocytes are used in the culture. One is phenylhydrazine (Phenylhydrazine) -induced: the uninfected CBA mice were given intraperitoneal hydrochloride phenylhydrazine 2.5mg, 8 days later, the mouse blood that contains 20 to 30% reticulocytes; the other said The “crisis-post” (post-crisis) who: that the mice vaccinated with the drug cleared Plasmodium, the blood that contains 40 to 60% of uninfected reticulocytes. Cultures were performed at 37 ° C and 20 ° C.