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随着对棕色脂肪组织(BAT)在成人体内具有生物学功能的肯定,棕色脂肪已经成为当今医学研究的热点。采用动物实验、病理组织学方法及免疫组化,观察了表没食子儿茶素没食子酸酯(EGCG)对棕色脂肪代谢的影响。结果表明,在体重和鼠龄相近时,不同种属小鼠肩甲间棕色脂肪组织HE染色后的形态不同,C57BL/6脂肪细胞中的空泡大,胞浆含量少,BALB/c小鼠脂肪细胞中的空泡小,胞浆含量多,昆明小鼠居中。给昆明小鼠口服不同剂量EGCG后,150 mg/kg EGCG能降低小鼠体重的增长,降低附睾周围白色脂肪组织的重量,但没有统计学意义。同时,EGCG能降低棕色脂肪细胞内脂肪含量,增加胞浆含量,具有统计学意义(P<0.001)。免疫组化结果表明,EGCG能增加棕色脂肪细胞内脱偶联蛋白1(UCP1)的表达,增加能量代谢。因此,不同种属小鼠的BAT具有不同的组织形态学特点,这为研究BAT小鼠的选择提供了依据;EGCG能够调节小鼠棕色脂肪的代谢功能,这为进一步研究作用机制打下了基础,同时为茶叶在脂肪代谢方面的调节提供了一种新的思路和依据。
With the affirmation of the biological function of brown adipose tissue (BAT) in adults, brown fat has become a hot spot in medical research today. The effects of epigallocatechin gallate (EGCG) on brown fat metabolism were observed by means of animal experiments, histopathological methods and immunohistochemistry. The results showed that the morphological changes of brown adipose tissue in the shoulder pad of different species were different when the body weight and the age of the mice were similar. The vacuoles in the C57BL / 6 adipocytes were large and the content of cytoplasm was small. BALB / c mice Small vacuoles in adipocytes, cytoplasm and more Kunming mice in the middle. After oral administration of different doses of EGCG to Kunming mice, 150 mg / kg EGCG reduced the body weight of mice and reduced the weight of white adipose tissue around the epididymis, but it was not statistically significant. At the same time, EGCG can reduce the fat content of brown adipocytes and increase the content of cytoplasm, with statistical significance (P <0.001). Immunohistochemistry showed that EGCG increased the expression of UCP1 in brown adipocytes and increased energy metabolism. Therefore, different kinds of mice BAT has different histomorphological characteristics, which provides a basis for the study of BAT mouse selection; EGCG can regulate the metabolic function of mouse brown fat, which laid the foundation for further study of the mechanism of action, At the same time, it provides a new idea and basis for tea’s regulation in fat metabolism.