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为研究大豆细胞壁脯氨酸富集蛋白基因在非生物胁迫下发挥的作用,利用实时荧光定量PCR,对大豆中的两个脯氨酸富集蛋白基因SbPRP1和SbPRP2在非生物胁迫下的表达情况进行检测。结果显示:SbPRP1在高盐和低温处理下表达量下降,分别在处理后1和24 h达到最低值;在模拟干旱处理下表达量先下降后升高,处理后24 h达到最大值。SbPRP2在高盐、模拟干旱和低温处理下表达量均有所升高,分别在处理后24,1和5 h达到最大值。表明SbPRP1和SbPRP2可能参与了大豆对不利环境的适应性调控。通过RT-PCR从大豆叶片中扩增SbPRP1和SbPRP2的基因全序列并构建到植物表达载体pRI101-AN上。
In order to study the role of proline-rich protein gene in soybean cell wall under abiotic stress, the expression of two proline-rich protein genes SbPRP1 and SbPRP2 in soybean under abiotic stress was detected by real-time fluorescence quantitative PCR Test. The results showed that the expression level of SbPRP1 decreased under high salt and low temperature treatment, reaching the lowest value at 1 and 24 h after treatment. The expression level of SbPRP1 decreased first and then increased and reached the maximum at 24 h after drought stress. The expression of SbPRP2 increased under high salinity, simulated drought and low temperature treatment, reaching the maximum value at 24, 1 and 5 h after treatment respectively. This indicates that SbPRP1 and SbPRP2 may be involved in the adaptive regulation of soybean on adverse environment. The full sequence of the genes for SbPRP1 and SbPRP2 was amplified from soybean leaves by RT-PCR and cloned into the plant expression vector pRI101-AN.