论文部分内容阅读
目的 : 探讨SLE患者免疫发病机理及其临床意义。方法 : 免疫印迹技术测定ENA抗体 ,免疫金标法测定ds DNA抗体 ,ELISA法测ANA、sCD10 6、sCD95、sCD2 5、sCD5 4。ESR按临床检测常规进行。结果 : 活动性SLE患者sCD10 6、sCD5 4、sCD2 5水平均高于非活动性SLE患者的相应值 (P <0 .0 5 ) ;而后者又分别高于正常对照组的相应值 (P <0 .0 5 ) ;活动期SLE患者sCD95水平高于非活动期或正常对照组 ,后两者sCD95水平无差别 (P >0 .0 5 )。相关分析表明 ,sCD10 6和sCD5 4相关 (r =0 .5 49,P <0 .0 5 ) ,sCD95与sCD2 5正相关 (r=0 .5 6 8,P <0 .0 5 ) ,ESR与sCD10 6、sCD5 4、sCD95及sCD2 5均相关 ,ANA只与sCD95相关 ,ds DNA在活动与非活动两组间差别明显 (x =4.76 ,P <0 .0 5 )与可溶性细胞表面抗原间无相关性。结论 : SLE患者自身免疫异常 ,可溶性sCD5 4、sCD95、sCD10 6及sCD2 5与活动性有关。
Objective: To investigate the pathogenesis of SLE and its clinical significance. Methods: The immunoblotting method was used to detect the ENA antibody, the immunogold assay was used to detect the dsDNA antibody, the ELISA method to test the ANA, sCD10 6, sCD95, sCD2 5, sCD5 4. ESR according to clinical testing routine. Results: The levels of sCD10 6, sCD5 4 and sCD2 5 in active SLE patients were significantly higher than those in inactive SLE patients (P <0.05), while the latter were higher than those in normal controls (P < 0. 05). The level of sCD95 in patients with active SLE was higher than that in inactive or normal controls, and there was no difference in sCD95 between the two groups (P> 0.05). Correlation analysis showed that there was a positive correlation between sCD10 6 and sCD5 4 (r = 0.549, P <0.05), sCD95 and sCD2 5 (r = 0.56, P0.05), ESR SCD95 and sCD2 5 were correlated with sCD10 6, sCD95 and sCD2 5, ANA was only associated with sCD95, ds DNA was significantly different between active and inactive groups (x = 4.76, P <0.05) and between soluble cell surface antigen No correlation. Conclusion: SLE patients with autoimmune abnormalities, soluble sCD5 4, sCD95, sCD10 6 and sCD2 5 and activity related.