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本研究利用籼稻品种Big Grain1(BG1)和粳稻品种Xiaolijing(XLJ)为亲本杂交建立的包含269个家系的重组自交系群体及相应的分子连锁图在杭州富阳(2011)和海南陵水(2012)两个自然环境条件下,对粒形和粒重性状进行了相关性分析及QTL定位分析,并对QTL上位性效应及与环境的互作进行了分析。分析表明,粒形和粒重性状间存在着相关性;QTL分析结果中共检测到34个QTL,其中与粒重性状相关的QTL有5个,与粒形性状相关的QTL有29个;它们分布在第2、第3、第4、第5、第6、第7和第9染色体上,贡献率介于3.22%~27.24%之间;此外,还检测到2个与环境互作的QTL和9对双因子互作。
In this study, 269 lines of recombinant inbred lines (IDHs) and corresponding molecular linkage maps were established in the hybrid rice of Big Grain1 (BG1) of Indica Rice and Xiaolijing (XLJ) of Japonica Rice in Hangzhou Fuyang (2011) and Hainan Lingshui (2012) ) Under two natural conditions, correlation analysis and QTL mapping of grain shape and grain weight traits were conducted. The epistatic effects of QTLs and the interaction with QTLs were also analyzed. The results showed that there were correlations between grain shape and grain weight traits. A total of 34 QTLs were detected in QTL analysis, including 5 QTLs associated with grain weight traits and 29 QTLs associated with grain traits. The contribution rates ranged from 3.22% to 27.24% on the second, the third, the fourth, the fifth, the sixth, the seventh and the ninth chromosomes. In addition, two QTLs interacting with the environment were detected 9 pairs of two-factor interaction.