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比较了荧光显微镜观察法，流式细胞仪检测法及ＴｄＴ末端标记法检测高能Ｘ线及加热诱导人鼻咽癌细胞系的凋亡指数。结果发现ＣＮＥ－２细胞系受照２、４、８、１２及２０Ｇｙ后三种检测方法的凋亡指数荧光法分别为１５％、２０％、３１％、３８％及４８％；流式法为１６．５％、２０．１％、３３％、４２．３％及４７．５％；ＴｄＴ法为２４．６％、３０．０％、４０．６％、５０．１％及５８．７％。我们使用ＴｄＴ法作为加热诱导凋亡的检测方法，发现于４１℃培养０．５、１、１．５、２．５ｈ后凋亡指数分别为１６．７％、２４．５％、３０．７％及４３％。结果提示ＴｄＴ末端标记法是比较敏感的检测方法，ＣＮＥ－２细胞系是对放、热疗均较敏感的实验模型
Fluorescence microscopy, flow cytometry and TdT-labeling were used to detect apoptosis index of high-energy X-ray and heat-induced human nasopharyngeal carcinoma cell lines. The results showed that apoptotic index fluorescence of CNE-2 cell line was 15%, 20%, 31%, 38% and 48% respectively after being exposed to 2,4,8,12 and 20 Gy. The flow cytometry 16.5%, 20.1%, 33%, 42.3% and 47.5% respectively; the TdT method was 24.6%, 30.0%, 40.6%, 50.1% and 58.7% . We used the TdT method as a heat-induced apoptosis detection method and found that the apoptotic index after incubation at 41 ° C for 0.5, 1, 1.5 and 2.5 h was 16.7%, 24.5% and 30.7, respectively % And 43%. The results suggest that the TdT-end labeling method is a sensitive detection method. CNE-2 cell line is an experimental model that is sensitive to release and hyperthermia