川贝母是常用中药,具有止咳化痰的功能,长期以来,供不应求。无性繁殖时,用种量大影响着川贝母生产。作者用组织培养的方法,达到快速繁殖,为生产提供种茎。以贝母[卷叶贝母Fritillaria cirrhosa]的鳞瓣,叶腋,叶片为材料,用组织培养方法获得小鳞茎,完整植株及愈伤组织。薄层层析的植化结果表明,再生小鳞茎与用于组培的原种鳞茎及市售青贝均有甾体皂甙类成分的反应。 Fritillaria cirrhosa is a commonly used traditional Chinese medicine, which has the function of relieving cough and phlegm. For a long time, it has been in short supply. When vegetatively propagated, the production of Fritillaria cirrhosa is influenced by the amount of seed. The authors used tissue culture methods to achieve rapid propagation and provide stems for production. Fritillaria cirrhosa (Fritillaria cirrhosa) was used as a material to obtain small bulbs, intact plants and callus in tissue culture. The phytochemical results of TLC indicated that the regenerated bulblet reacted with the steroidal saponin component of the parent bulb used for tissue culture and the commercially available green shellfish.